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Ozone Test Kits

Visual Kit

Range MDL Method Type Kit Cat. No. Refill Cat. No.
0.0 - 0.6 & 0.6 - 3.0 ppm 0.025 ppm DPD CHEMets K-7404 R-7404

Instrumental Kits

Range Method Type Kit Cat. No.
0 - 0.75 ppm Indigo Vacu-vials K-7433
0 - 0.75 ppm Indigo SAM Photometer + Vacu-vials I-2022
0 - 5.00 ppm DPD Vacu-vials K-7423
0 - 5.00 ppm DPD SAM Photometer + Vacu-vials I-2019

The CHEMetrics test kits for the determination of Ozone in aqueous solutions are based on patented Self-Filling Reagent Ampoule technology, and employ the Indigo and DPD methods. Premixed. Premeasured. Precise. Each kit contains 30 tests. Suitable for industrial water testing as well as food safety and aseptic bottling testing.

The Ozone Vacu-vials test kit K-7423 can be used with a Hach DR900 Colorimeter in conjunction with the CHEMetrics DR900 Vacu-vials® Adapter, Cat. No. A-0215. No endorsement by Hach Company is implied or intended.

The DPD Method

Potassium iodide is added to the sample before analysis. Ozone reacts with the iodide to liberate iodine. The iodine reacts with DPD (N, N-diethyl-p-phenylenediamine) to form a pink colour. Results are expressed as ppm (mg/l) O3.

USEPA Methods for Chemical Analysis of Water and Wastes, Method 330.5 (1983).
APHA Standard Methods, 22nd ed., Method 4500-Cl G- 2000.

Technical Data Sheet

The Indigo Method

With the indigo method, indigo trisulphonate dye immediately reacts with ozone. The colour of the blue dye decreases in intensity in proportion to the amount of ozone present in the sample. The test reagent is formulated with malonic acid to prevent interference from up to at least 10 ppm chlorine. Results are expressed as ppm (mg/l) O3.

The CHEMetrics Indigo Ozone Vacu-vials® Kit employs an innovative "self-zeroing" feature to eliminate the need to generate a reagent blank. Each Vacu-vials® ampoule is measured before and after being snapped in sample. The change in colour intensity, measured in absorbance between reagent in the unsnapped and snapped ampoule is used to determine the ozone concentration of the sample. Only one ampoule is required per test, meaning shorter testing times, greater efficiency and cost savings.

The Indigo Vacu-vials kit is compatible with a number of different spectrophotometers that accept a 13 mm diameter round cell, and with a 'Z dimension' of 15mm or less (because of the self-zeroing feature).

Click here for more information on CHEMetrics Ozone Indigo test kits and bottle water testing, and an Ozone Indigo product comparison.

Bader H. and J. Hoigné, "Determination of Ozone in Water by the Indigo Method," Water Research Vol. 15, pp. 449-456, 1981.
APHA Standard Methods, 22nd ed., Method 4500-03 B (1997).

Technical Data Sheet


Ozone (O3) is an inorganic molecule containing three oxygen atoms. It is an allotrope of oxygen (O2) although much less stable. Ozone is a pale blue gas with a distinctive chlorine-like smell and slightly soluble in water. Ozone is a powerful oxidising agent, much more so than Oxygen. It is found in low concentrations in the Earth's stratosphere and can be produced by electrolysis or corona discharge.

Ozone's mechanism of action is to destroy microorganisms through oxidation, for which microorganisms cannot build up a resistive tolerance to, rather than as systematic poisoning. Ozone is a broad-spectrum biocide, and can also help to control algae and other aquatic growths. One of the advantages of ozone is that it breaks down to oxygen within a short period of time leaving no residual chemicals or contaminants. Its use therefore does not require additional rinsing steps.

Ozone is used as an alternative to chlorine, as a broad-spectrum biocide, disinfectant and steriliser. Ozone is used in a variety of industries and processes, including air and water purification, deodourisation, decolourisation, food safety and pharmaceutical industries. It has particular relevance to aseptic bottling in the bottled water industry.