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Hydrogen Peroxide Test Kits

Visual & Titrimetric Kits

Range MDL Method Type Kit Refill
0.0 - 0.5 ppm 0.025 ppm DPD CHEMets K-5502 R-7904
0.0 - 0.8 & 1 - 10 ppm 0.05 ppm Ferric Thiocyanate CHEMets K-5510 R-5510
5- 50 ppm 5 ppm Ferric Thiocyanate HR CHEMets K-5520D R-5510
25 - 250 ppm 25 ppm Ferric Thiocyanate HR CHEMets K-5520A R-5510
125 - 1,250 ppm 125 ppm Ferric Thiocyanate HR CHEMets K-5520B R-5510 + A-0171
1,000 - 10,000 ppm 1,000 ppm Ferric Thiocyanate HR CHEMets K-5520C R-5510 + A-0171
0.1 - 1.0 % (2 - 20 % w/dilution)* 0.10% Ceric Sulphate Titrant / Ferroin Titrets K-5530 -

Instrumental Kits

Range Method Type Kit
0 - 6.00 ppm Ferric Thiocyanate Vacu-vials K-5543
0 - 6.00 ppm Ferric Thiocyanate SAM Photometer + Vacu-vials I-2016

The CHEMetrics test kits for the determination of Hydrogen Peroxide in aqueous solutions employ three different test methods, DPD, Ferric Thiocyanate and Ceric Sulphate, delivering sensitivity and accuracy in two minutes or less. Based on CHEMetrics patented Self-Filling Reagent Ampoule technology. Premixed. Premeasured. Precise. Each kit contains 30 tests. Visual, instrumental and titrimetric H2O2 testing kit formats span low and high measurement ranges.

CHEMets® visual test kits use colour comparators for analysis. CHEMets test kit K-5502 utilises the DPD chemistry to measure H2O2 from 0 to 0.5 ppm. Several visual kits that employ the ferric thiocyanate chemistry are available for determination of hydrogen peroxide at low and high concentration ranges.

Vacu-vials® instrumental kits rely on CHEMetrics direct-readout photometers or spectrophotometers capable of accepting a 13-mm diameter round cell. The Vacu-vials kit K-5543 utilise the ferric thiocyanate chemistry and can be optionally used with a Hach DR900 Colorimeter in conjunction with the CHEMetrics DR900 Vacu-vials® Adapter, Cat. No. A-0215. No endorsement by Hach Company is implied or intended.

Titrets® offer a unique reverse titrimetric method. The Titrets test kit K-5530 uses ceric sulphate titrant with ferroin indicator. Depending on the procedure used, this kit can determine H2O2 concentrations from 0.01% to as high as 20%.

CHEMetrics also offers a 0.5 ppm hydrogen peroxide analytical standard A-5505 that can be used to verify kit reagent and instrument performance reliability.

The Ferric Thiocyanate Method

The ferric thiocyanate method consists of ammonium thiocyanate and ferrous iron in an acidic solution. Hydrogen peroxide oxidises ferrous to ferric iron, resulting in the formation of a red thiocyanate complex. Chlorine does not interfere with this method, but Ferric iron does. Results are expressed as ppm (mg/l) H2O2.

Reference:
D. F. Boltz and J. A. Howell, eds., Colorimetric Determination of Nonmetals, 2nd. ed., Vol. 8, p. 304 (1978).

Technical Bulletin:
Hydrogen Peroxide Analysis in the Presence of Peracetic Acid and Copper
Technical Data Sheet

The DPD Method

With the DPD Method, hydrogen peroxide in the sample reacts with DPD (N, N-diethyl-p-phenylenediamine) in the presence of potassium iodide and ammonium molybdate to form a pink colour in the ampoule. Results are expressed as ppm (mg/l) H2O2.

References:
USEPA Methods for Chemical Analysis of Waters and Wastes, Method 330.5 (1983).
APHA Standard Methods, 21st ed., Method 4500-Cl G (2005).
D.F. Boltz and J.A. Howell, eds., Colorimetric Determination of Nonmetals 2nd ed., Vol. 8, p. 303 (1978).

Technical Data Sheet

The Ceric Sulphate Titrimetric Method

CHEMetrics has developed a patented reverse titrimetric method using ceric sulphate as the titrant and ferroin as the end point indicator. A colour change from green to orange signals the end of the titration. Results are expressed as percent (%) H2O2. The test range can be also be modified by performing a sample dilution. Details are provided in the kit instructions for testing samples in the ranges of 0.01 - 0.1% through 2- 20%.

* Up to 20% with dilution

Reference:
Developed by CHEMetrics.

Technical Data Sheet

Comparison with EMD Millipore's MQuant Test Strips

This CHEMetrics Report examines statistical data from 2018 for 2 CHEMetrics Hydrogen Peroxide Test Kits, CHEMets® K-5510 Visual Test Kit and K-5543 Vacu-vials® Photometric Test Kit, and EMD Millipore's MQuant™ Test Strips (Part No. 110011). The K-5543 was measured using 2 CHEMetrics photometers, I-2016 Dedicated Photometer (SAM) and V-2000 Multi-Analyte Phometer. 4 H2O2 standard concentrations were measured, 0, 0.15, 0.25 and 0.50 ppm. Whilst the CHEMetrics kits were able to distinguish concentrations in 0.05 ppm increments and an accuracy of ± 0.02 ppm respectively, MQuant™ Test Strips had only 2 pale colour intervals between 0 & 0.5 ppm, and whilst it was possible to distinguish between 0, 0.25 and 0.5 ppm, colour development was barely perceptible at 0.15ppm.

Comparative Performance Study:
CHEMetrics® Hydrogen Peroxide Self-filling Ampoules vs Test Strips

Standard Solution

CHEMetrics® offers a standard solution for hydrogen peroxide, catalogue no. A-5505, for the preparation of 0.5 ppm standard, offers the analyst an easy-to-use, NIST Traceable standard to confirm the accuracy and reliability of hydrogen peroxide measurements for test kits K-5502, K-5510 and K-5543.

Applications

Hydrogen peroxide finds use in many different industries due to its strong oxidising properties and its disinfectant properties. For the pulp and paper industry, it is used as a bleaching agent. In municipal drinking water, wastewater and water treatment industries, H2O2 is utilised to control taste and odour, and to remove hydrogen sulphide and iron. It is also used in the treating of industrial effluents and solid waste processing. Various forms of advanced oxidation processes rely on H2O2 to effectively remediate environmental contamination.

In the food and beverage industry, hot H2O2 mists are used as a sterilant in aseptic and extended shelf-life packaging (ESL) equipment as the packages are formed in line, in order to eliminate microbial pathogenic contamination. The product cartons are sprayed with hydrogen peroxide to pre-sterilise them, and then heated or pressed to remove the hydrogen peroxide. A hydrogen peroxide solution is also used to clean and sterilise manufacturing equipment used in the aseptic packaging processing line. However, H2O2 residuals must then be removed before the filling process to prevent negative impacts to the products’ taste and nutritional profiles. CHEMetrics H2O2 CHEMets® and Vacu-vials® test kits are used extensively in the food and beverage industry for this purpose. The US FDA specifies a limit on H2O2 residual in packaging of no more than 0.5 ppm (mg/l) (w/v) according to FDA guidelines (21 CFR 178.1005). This H2O2 level is often referenced globally as an acceptable residual limit.

What is Hydrogen Peroxide?

Peroxides are chemicals with an oxygen to oxygen bond (R-O-O-R, where R is an element and O-O is the oxygen bond). Hydrogen peroxide (H2O2) is the simplest peroxide. It is a colourless or pale blue liquid at room temperature, depending on concentration, that breaks down into the by-products of water and oxygen. H2O2 is a strong oxidising agent.