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Detergents Test Kits

Visual Kit

Range MDL Method Type Kit Cat. No. Refill Cat. No.
0.0 - 3.0 ppm 0.125 ppm Methylene Blue CHEMets K-9400 R-9400

Instrumental Kits

Range Method Type Kit Cat. No.
0 - 2.50 ppm Methylene Blue Vacu-vials R-9423
0 - 2.50 ppm Methylene Blue SAM Photometer + Vacu-vials I-2017

The CHEMetrics test kits for for the determination of Detergents (anionic surfactants, MBAS) in aqueous solutions are based on patented Self-Filling Reagent Ampoule technology, and employ the Methylene Blue method. Premixed. Premeasured. Precise. Each CHEMets kit contains 30 tests (Vacu-vials contain 20 tests). Suitable for pharmaceutical industry as well as residential water testing.

The Methylene Blue Method

The methylene blue active substances (MBAS) method is used in a 3-minute procedure to measure anionic detergents in the 0-3 ppm (mg/l) range. The procedure features a unique extraction/sampling technique that eliminates several steps required in other test procedures and provides increased sensitivity.

Anionic detergents react with methylene blue to form a blue-coloured complex that is extracted into an immiscible organic solvent. Results are expressed in ppm (mg/l) as linear alkylbenzene sulphonate (LAS), equivalent weight 325.

USEPA Methods for Chemical Analysis of Water and Wastes, Method 425.1 (1983).
APHA Standard Methods, 21st ed., Method 5540 C (2005).
ASTM D 2330-02 , Methylene Blue Active Substances.

Technical Data Sheet


Detergents can be introduced into the water supply by industry, soap manufacturers, and private households. Environmental analysts often include a determination of anionic detergents when assessing surface water pollution.

Cleaning validation is a requirement in industries such as pharmaceutical manufacturing which adhere to Good Manufacturing Practice (GMP) and Quality Systems Regulations (QSR), and is specific to the cleaning method and agent employed. A cleaning validation involves testing for acceptable residue on pharmaceutical manufacturing or medical device surfaces. CHEMetrics® Detergents Test Kit, Cat. No. K-9400, featuring a superior extraction/sampling technique, is routinely used by pharmaceutical companies to measure detergents (anionic surfactants) to ensure that no excessive residual surfactant remains.